Objective To evaluate the effectiveness of hand hygiene (HH) compliance management system in the control of healthcare-associated infection (HAI). Methods From July 2020 to June 2021, staff in the respiratory intensive care unit of a tertiary first-class hospital were selected as research objects. Changes in staff's HH compliance rate and HAI management indicators before and after the application of HH compliance management system were observed and analyzed. Results After applying HH compliance management system, overall HH compliance rate and HH compliance rates of staff at different job positions (including doctors, nurses, and cleaners) improved significantly (P < 0.05). Incidence of HAI and HAI with multidrug-resistant organisms (MDROs) in the trial group were significantly lower than those in the control group (P < 0.05). Conclusion The application of HH compliance management system can effectively increase staff's HH compliance rate and reduce the occu-rrence of HAI.