Objective To construct a quantitative evaluation system for professional competency of healthcare-associated infection (HAI) management professionals, and provide scientific basis for the training of professionals through scientific evaluation. Methods Literature and experience summary were adopted to construct evaluation key points. Evaluation key points were selected through expert consultation method, and the weight coefficient of evaluation key points was calculated. The hierarchical evaluation content options for each evaluation key point were designed and assigned score values by expert group. The product of the score of each evaluation key point and its weight was the score for this point, and the total score of the evaluated person was calculated based on the total score of all evaluation points. Results The evaluation system included 25 evaluation key points in 9 dimensions, including "basic conditions" "recognition competency in HAI" "monitoring competency in HAI" "prevention and control technology application competency in HAI " "emergency response competency" "organization and coordination competency" "quality improvement competency" "education and training competency" and "professional scientific research competency in HAI prevention and control". Each evaluation key point contained three hierarchical quantitative score contents. The internal consistency reliability of the expert's questionnaire consultation Cronbach's α coefficient was 0.873, the total scale-level content validity index (S-CVI) was 0.868, and the item-level content validity index (I-CVI) ranged 0.71-1. Conclusion The quantitative evaluation system constructed in this study meets the requirements for reliability and validity. It is scientifically feasible for evaluating the professional competency of HAI management professionals. It can effectively identify weakness in their competency, determine training directions and priorities, and provide a scientific basis for talent development and team building for HAI management profe-ssionals in medical institutions, and promotes high-quality development within hospitals.