Objective To evaluated the effect of doctor-nurse integrated infection control management in operating room on prevention and control of healthcare-associated infection (HAI) in patients undergoing ophthalmic surgery. Methods 4612 patients with ophthalmic surgery from August 2019 to January 2020 were selected as the control group and implemented routine medical management mode; 4905 patients with ophthalmic surgery from May to October 2020 were selected as the intervention group and implemented doctor-nurse integrated infection control mana-gement mode, HAI prevention and control attitude and behavior of health care workers (HCWs) before and after the intervention as well as HAI after ophthalmic surgery were investigated and analyzed. Results HAI rates of patients before and after the implementation of doctor-nurse integrated infection control management were 1.22‰ (n=6) and 0.22‰ (n=1) respectively, there was significant difference between two groups (χ²=3.892, P=0.049). After the implementation of integrated infection control management mode, scores of HCWs' HAI prevention and control attitude on mask wearing, hand hygiene, aseptic operation, medical waste disposal and occupational protection were all higher than those before the intervention, scores of HCWs' HAI prevention and control behavior on mask wearing, hand hygiene, aseptic operation, medical waste disposal, occupational protection and operating room environmental management were all higher than those before the intervention (all P < 0.05). Conclusion The doctor-nurse integrated infection control management can effectively decrease HAI in patients undergoing ophthalmic surgery, improve the attitude and behavior of HCWs towards HAI prevention and control.