Objective To investigate Candida auris(C. auris) infection/colonization in patients and environmental contamination in intensive care unit (ICU) of a hospital, and evaluate the effect of healthcare-associated infection(HAI) prevention and control measures. Methods In June 2022, C. auris was isolated from the blood culture of a long-term hospitalized patient in the ICU of a hospital. Multiple sampling and bacterial culture (once for every two weeks) were immediately performed on different sites of patient's body surface and ward environment. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was adopted for analysis. For patients with C. auris, measures such as single room isolation, warm water bathing, chlorhexidine scrubbing were taken. Ward environment was wiped and disinfected. The effect of prevention and control measures was evaluated by active screening. Results 14 patients in ICU were actively screened initially, 9 C. auris strains were isolated from 8 patients. C. auris was not detected in the 3 patients who were transferred out of ICU one week before the first detection of C. auris. 2 C. auris strains were isolated from 2 patients in the second active screening of 13 patients in ICU. In the third active screening of 8 patients in ICU, C. auris was found in the groin and armpit of 1 patient, other patients were all negative. The fourth and fifth active screening didn't detect C. auris. Environmental monitoring result showed that 6 C. auris strains were isolated from the ground and medical equipment in the first active screening of 80 environmental specimens. No C. auris was detected in the second and third screening. After taking comprehensive prevention and control measures, only 1 of the 14 patients suffered from healthcare-associated septicemia due to C. auris. The rest patients were confirmed with C. auris colonization and decolonized at the late stage. Conclusion C. auris is easy to spread in hospital wards, but can be prevented and controlled by taking active HAI prevention and control measures.