Objective To explore the effect of bundled care on reducing the incidence of pulmonary infection (PI) in patients with acute leukemia receiving chemotherapy. Methods Quasi-experimental research design was adopted, and patients with acute leukemia in the department of hematology in a tertiary first-class hospital from August 2019 to December 2021 were chosen as the study subjects. Ward A was the control group, and Ward B was the intervention group. The intervention group and the control group were matched based on the type of leukemia and age (±5 years). The intervention group received bundled care intervention measures, including systematic health education led by nurses, reducing exposure, enhancing oral care, and pulmonary function exercises driven by respiration trai-ning device. The control group received routine measures. Two groups were continuously observed for two chemotherapy courses, and the differences in PI rates, PI types, and pulmonary function (the percentage of forced expiratory volume in one second[FEV1%]) between two groups of patients were analyzed and compared. Results In the first chemotherapy course, 47 pairs of patients from two groups were matched, and 34 pairs of patients ultimately matched in the second chemotherapy course. Incidence of PI in patients in the intervention group and the control group were 35.80% and 43.21%, respectively, with no statistically significant difference (P>0.05). The incidence of invasive pulmonary fungal infections was 3.70% in patients in the intervention group and 12.35% in the control group, with a statistically significant difference (P < 0.05). Repeated measures analysis of variance showed that the FEV1% value in patients in the intervention group was better than that in the control group (P < 0.05). The incidence of oral mucositis in patients in the intervention group was 11.11%, which was statistically lower than 24.69% in the control group (P < 0.05). Conclusion Bundled care can effectively reduce the incidence of pulmonary fungal infections in patients and improve patients' pulmonary function, while preventing the occurrence of oral mucositis. However, further investigation is needed to explore its effect on reducing the incidence of other types of PI.