Objective To retrospectively analyze the distribution and antimicrobial resistance of Listeria monocytogenes (L. monocytogenes) in 75 hospitals using the data from Hebei Provincial Bacterial Resistance Monitoring System from 2013 to 2022, and to provide a basis for clinical treatment and rational use of antimicrobial agents. Methods Specimen sources, department distribution, and antimicrobial resistance of L. monocytogenes isolated from clinical cases in 75 hospitals in Hebei Province from January 2013 to June 2022 were analyzed with WHONET software. Results From 2013 to 2022, a total of 187 strains of L. monocytogenes were isolated in Hebei Province. The specimens were mainly blood (129 strains, 69.0%), cerebrospinal fluid (20 strains, 10.7%), gastric fluid (14 strains, 7.5%), and others (18 strains, 9.6%). 70 (37.4%) strains were isolated from males, and 117 (62.6%) were from females. Department of neonatology (42 strains, 22.5%) as well as department of obstetrics and gynecology (41 strains, 21.9%) were the most common sources. In terms of age groups, young adults had the highest isolation rate (74 strains, 39.6%), followed by neonates (39 strains, 20.9%). The susceptibility rates of the strains to penicillin, ampicillin, meropenem, compound sulfamethoxazole and erythromycin were 97.8%, 98.6%, 98.0%, 98.4% and 96.3%, respectively. Conclusion From 2013 to 2022, most L. monocytogenes strains isolated from clinical cases in Hebei Province were from blood specimen, and the infected population were mainly young adults and neonates. Non-susceptible strains to first-line antimicrobials have emerged and continuous attention is needed.