Objective To explore the application effect of multi-disciplinary team (MDT) model in hospital antimicrobial management. Methods Relevant data on antimicrobial use in hospitalized patients in a hospital from January 2021 to December 2022 were analyzed retrospectively, January-December 2021 adopted conventional management mode and was as the control group, January-December 2022 adopted MDT management model and was as the intervention group. Antimicrobial therapy relevant indicators between two groups of patients were compared. Results After adopting the MDT management model, pathogen detection rate before the therapeutic antimicrobial use in the intervention group (73.62%) was higher than that in the control group (70.56%), difference was statistically significant (P < 0.001). Pathogen detection rate related to healthcare-associated infection diagnosis was 87.98% in the control group and 88.89% in the intervention group, with no statistically significant difference between two groups (P>0.05). Pathogen detection rate before combined use of key antimicrobial agents in the intervention group (93.94%) was higher than that in the control group (92.00%), difference was statistically significant (P < 0.05). Antimicrobial use rate in hospitalized patients and use rate of prophylactic antimicrobial agents in class Ⅰ incision surgery decreased from 38.03% and 21.03% to 32.78% and 10.30%, respectively, with statistically significant differences (both P < 0.05). The amount and intensity of antimicrobial use in hospitalized patients in the intervention group decreased. The implementation rate of bundled prevention and control measures for multidrug-resistant organism (MDRO) after intervention was significantly higher than that of the control group, with statistically significant differences (all P < 0.05). MDRO detection rate decreased from 34.70% to 32.37%, difference was statistically significant (P=0.027). there was no significant change in the MDRO case infection rate. Conclusion The MDT model can effectively improve the standardized management of antimicrobial agents, promote the rational use of antimicrobial agents in clinical practice, and prevent bacterial resistance.