Objective To analyze the distribution and antimicrobial resistance of pathogens from wounds of burned patients, providing reference for the rational use of antimicrobial agents and healthcare-associated infection (HAI) prevention and control. Methods Clinical data of burned patients admitted to a tertiary first-class hospital from Ja-nuary 2020 to December 2022 were analyzed retrospectively, pathogens in the wound was cultured, identified, and performed antimicrobial susceptibility analysis. Results From 2020 to 2022, a total of 588 burned patients were admitted, 734 strains of pathogens were detected, including 415 strains (56.54%) of Gram-negative bacteria, 306 strains (41.69%) of Gram-positive bacteria, and 13 (1.77%) strains of fungi. The top 5 pathogens were Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterobacter cloacae. Staphylococcus aureus had higher resistance rates (93.02%-97.37%) to penicillin G, resistance rate to oxacillin increased from 11.63% to 21.92%. Pseudomonas aeruginosa mainly exhibited resistance to ticarcillin/clavulanic acid, aztreonam, and levofloxacin, resistance rates to imipenem and meropenem were 15.00% -38.10% and 10.00%-33.33%, respectively. Susceptibility of Enterobacterales bacteria to cephalosporins enhanced with the increased of cephalosporin generations, and exhibited higher resistance to commonly used antimicrobial agents. Conclusion Over the past three years, there has been no significant change in the detection of major pathogens and antimicrobial resistance in wounds of burned patients in this hospital. Antimicrobial resistance of Staphylococcus aureus and Enterobacterales is relatively severe, and it is necessary to carry out surveillance on pathogens from burn wounds in corresponding areas.