Objective To investigate the current status and influencing factors of nurses’ missed nursing care in infection prevention and control, and provide reference for establishing targeted programs to improve nursing care situation in clinical infection prevention and control. Methods From October to November 2023, 369 nurses from two tertiary first-class hospitals in Zhejiang Province were selected as surveyed subjects by convenience sampling methods. General information questionnaire, missed nursing care in infection prevention and control survey, hospital ethical climate survey, and safety attitudes questionnaire were used for questionnaire survey. Univariate analysis, Pearson correlation analysis, and multiple linear regression analysis were adopted to analyze the influencing factors. Results A total of 364 valid questionnaires were collected, with a respond rate of 98.64%. The scores of 364 nurses’ missed nursing care in infection prevention and control survey B and C were (48.94±15.06) and (47.88±14.54) points, respectively. The score of hospital ethical climate was (103.00±16.01) points, and the score of safety attitude was (128.50±17.00) points. Multiple linear regression analysis showed that the influencing factors for missed nursing care in infection control and prevention were temporary employment modes, work at pre-hospital emergency departments, and low scores in hospital ethical climate (all P<0.05). Conclusion Nurses’ missed nursing care in infection prevention and control is at a lower-middle level, there is still room for improvement. Nur-sing managers should reduce missed nursing care in infection prevention and control by improving the hospital ethical climate and taking appropriate intervention measures for nurses in different departments and employment styles.