Objective To explore the clinical characteristics as well as infection prevention and control strategies of patients with Candida auris (C. auris) infection/colonization. Methods Clinical characteristics as well as prevention and control measures of patients detected with C. auris in a general hospital from September 2023 to March 2024 were analyzed, prevention and control measures were evaluated. Results A total of 9 patients were detected C. auris, and the specimen sources were urine from 5 cases (55.56%), sputum from 3 cases (33.33%), and abdominal drainage fluid from 1 case (11.11%). There were 5 cases of community-associated infection (55.56%), 3 cases of community colonization (33.33%), and 1 case of hospital colonization (11.11%). There were 5 cases of deaths (55.56%), 3 cases of discharges (33.33%), and 1 case of transfer (11.11%). Through measures such as training, early warning, single-room isolation, personal protection, hand hygiene, environmental cleaning and disinfection and strengthening supervision on sampling process, supervising sampling detection and screening, terminal disinfection, and ensuring a qualified detection result of specimens before admitting new patients, no C. auris was detected from patient’s surrounding environment sampling, and no cross-transmission occurred in the hospital. Conclusion Paying attention to the implementation of prevention and control measures for C. auris infection can effectively control the occurrence and cross transmission of healthcare-associated infection.