Objective To analyze the research hotspots and trends of invasive fungal infections (IFIs) in China over the past 24 years, and provide references and suggestions for future research. Methods Literatures on IFIs research in China from 2000 to 2024 were retrieved from China National Knowledge Infrastructure (CNKI) and Web of Science (WOS). CiteSpace software was employed to conduct collaboration network analysis on authors’ institutions, and co-occurrence, clustering, and burst analyses were conducted on key words. Results A total of 2 479 li-teratures retrieved from CNKI and 1 149 from WOS were included in the analysis, involving 295 research institutions, with no core research team identified having a intermediary centrality >0.1. Institutions with intermediary centrality >0.1 included Chinese Academy of Sciences, Peking University, Fudan University, Sun Yat-sen University and Zhejiang University. Key words with high centrality included "diagnosis" "risk factor" "voriconazole" and "Candida albicans". Clustering analysis grouped the co-occurrence network of key words into 6 clusters, mainly covering fungal diagnosis, treatment, and susceptible factors. Since 2020, focuses on the safety of antifungal treatment agents and primary prevention have emerged, with hot topics including pharmacokinetics, children, clinical features, and risk factors. Conclusion Currently, research teams are dispersed with insufficient interdisciplinary collaboration. Research topics are relatively simple. It is necessary to strengthen research on fungal resistance and healthcare-associated infection prevention and control. Risk factors and prevention measures for IFIs may be the focus of future research.