Objective To investigate the contamination status of cloth mouse pads in dental clinics, and provide theoretical basis for standardizing environmental surface disinfection and scientific intervention in dental clinics. Methods The contamination status of cloth mouse pads in 5 dental clinics were investigated by adenosine triphosphate (ATP) fluorescence detection method, visual observation method, and bacterial culture method. Disinfection efficacy of ultraviolet disinfection method and wiping disinfection using two types of disinfectant wipes on cloth mouse pads and photovoltaic glass mouse pads was compared. Intervention measures (using photovoltaic glass mouse pads, regular cleaning and disinfecting) were taken, and contamination status of cloth mouse pads in different departments before and after intervention was compared. Results The baseline investigation found that the conta-mination status of mouse pads varied in different dental clinics. The qualified rate of bacterial culture of mouse pads in department of endodontics was the lowest (20.00%), followed by the department of periodontology (30.00%). There was statistically significant difference in ATP detection values between two types of disinfectant wipes before and after wiping and disinfecting photovoltaic glass mouse pads (both P<0.001). After using photovoltaic glass mouse pads, the contamination of mouse pads in 5 dental clinics was alleviated, and the qualified rate of ATP detection increased (＞80%), compared with qualified rate of ATP detection before intervention, differences were all statistically significant (all P<0.001). Conclusion The contamination of the cloth mouse pads in dental clinics is serious, and the conventional cloth mouse pad is difficult to be cleaned and disinfected. It is suggested that medical institutions use the mouse pad which is convenient for cleaning and disinfection, and specify the disinfection method and frequency to control the contamination of the mouse pads.