Objective To explore the distribution characteristics and influencing factors of nasal colonized bacteria of healthcare workers (HCWs) in pediatric intensive care unit (PICU). Methods A cross-sectional study was conducted. Nasal swab specimens from 104 HCWs in the PICU of a hospital were collected for bacterial culture and antimicrobial susceptibility testing. According to the identification and antimicrobial susceptibility testing results of strains, distribution characteristics of colonized bacteria was analyzed. Basic information of studied subjects were collected through questionnaire survey, and risk factors for colonized bacterial infection were conducted using logistic regression analysis. Results Among 104 specimens, colonized bacteria were detected from 66 specimens, with an overall detection rate of 63.46%. Gram-positive bacteria was mainly Staphylococcus aureus, with a detection rate of 34.62% (n=36), out of which methicillin-resistant Staphylococcus aureus (MRSA) accounted for 2.88% (n=3). Gram-negative bacteria was mainly Klebsiella spp., with a detection rate of 21.15% (n=22). Multiva-riate logistic regression analysis showed that HCWs with junior professional titles (OR=11.400, 95%CI: 2.329-55.801, P=0.003) was an independent risk factor for Staphylococcus aureus colonization, and male (OR=4.260, 95%CI: 1.160-15.653, P=0.029) was an independent risk factor for Klebsiella spp. colonization. Conclusion Nasal cavity of HCWs in PICU has a high detection rate of colonized bacteria, with Staphylococcus aureus and Klebsiella spp. being the major colonized bacteria.