Objective To evaluate the isolation rate and antimicrobial resistance of nonfermenters causing nosocomial infection in ICU in a hospital. Methods The bacteria were identified by VITEK-AMS, and the antimicrobial susceptibility tests were done according to the disc agar diffusion methods. Results The isolation rate of nonfermenters in ICU was 38.36%, the most common bacterium was P.aeruginosa ( 18.75%), the next was A.baumannii ( 11.18%) and S.maltophilia ( 4.38%). The bacterial isolation rates were different among different sites, respiratory system and skin incision had the highest bacterial isolation rate, which was 65.40% and 51.23% respectively. P.aeruginosa, A.baumannii and S.maltophilia had high antimicrobial resistant rate. Conclusion The isolation rate of nonfermenters were rather high in the nosocomial infection in patients of ICU, the resistant rates of nonfermenters to the antimicrobials were high, the drugs for the treatment of the infection caused by nonfermenters should be chosen according to the result of the antimicrobial susceptibility test.