ObjectiveTo study the influence of different load patterns on pressure steam sterilization parameters of autoclave.MethodsThermologgers was used to measure the temperature distribution of sterilization chamber without load, the temperature time variation at the center and on the upper layer of the tested package, as well as the pressure time variation of different load patterns. The influence of different load patterns on the temperature and air expelling level inside the tested package was analyzed.ResultsLoad pattern directly acts on the temperature and air expelling level inside sterilization package. Extremely heavy load pattern may lead to the temperature inside package being lower than required sterilization temperature and remain air inside package.ConclusionLoad pattern of autoclave can influence sterilization efficacy, it is necessary to standardize the load pattern of autoclave.