HIV感染者Th17和Treg细胞的变化及意义
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何艳

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R512.91

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国家十一五重大科技专项(2008ZX10001-008)


Significance of change in Th17 and Treg cells in HIVinfected patients
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    摘要:

    目的观察人免疫缺陷病毒(HIV)感染者辅助性T细胞(T help cell,Th)17和调节性T细胞(regulatory T cell, Treg)的数量、平衡状态变化及其意义。方法选取确诊的106例HIV感染者和30例健康体检者(健康对照组),采集静脉血,以流式细胞术检测CD4+T细胞及Th17细胞与Treg细胞百分比,RTPCR检测HIV RNA病毒载量。结果HIV感染者CD4+T细胞为13~702个/μL,分为4组,即A组:≤200个/μL, 19例;B组:201~350个/μL ,32例;C组:351~500个/μL,26例;D组:>500个/μL ,29例;上述各组均明显低于健康对照组(798~1 130个/μL)(P<0.05)。A、B、C、D组Th17细胞在CD4+T细胞中的百分比分别为(0.73±0.21)%、 (1.52±0.33)%、(2.92±0.46)% 、(3.74±0.58)%,均低于健康对照组的(4.90±1.43)%(P<0.05);A、B、C、D 组及健康对照组Treg细胞在CD4+T细胞中的百分比分别为(9.78±2.15)%、(7.23±1.72)%、(5.69±1.67)%、(4.62±0.97)% 、(4.43±0.97)%,除D组与健康对照组之差异无统计学意义(P>0.05),其余组均高于健康对照组(P<0.05)。HIV感染者病毒载量水平为(0~5.36)×107拷贝/mL,平均(5.36±1.21)×106拷贝/mL;A、B、C、D组的病毒载量分别为(6.24±1.36)×107拷贝/mL、(4.36±1.02) ×106拷贝/mL、(5.78±1.67)×104拷贝/mL、(3.54±1.25) ×103 拷贝/mL,各组间比较,差异有统计学意义(P<0.05),且与CD4+T细胞计数呈负相关。结论HIV感染导致Th17和CD4+T细胞下降、Th17和Treg细胞失衡,与HIV RNA载量负相关;与疾病进展密切相关。

    Abstract:

    ObjectiveTo evaluate the significance of change in T help cell 17(Th17) and regulatory T cell (Treg) in HIVinfected patients. Methods106 HIVinfected patients and 30 healthy volunteers were selected. Flow cytometry was used to detect the CD4+ T ,Th17 and Treg cells in peripheral blood. HIV RNA levels in serum were tested by RTPCR.ResultsCD4+ T cells were 13-702/uL, 19 patients were ≤200/uL (group A), 32 were 201-350/uL( group B), 26 were 351-500/uL(group C), 29 were >500/uL(group D); All were significantly lower than (798-1 130/uL) in healthy control group(P<0.05). The percentage of Th17 cells in CD4+ T cells were (0.73±0.21)%,(1.52±0.33)%, (2.92±0.46)% , and (3.74±0.58)% in group A, B ,C and D, respectively, which were all lower than (4.90±1.43)% of control group(P<0.05); The percentage of Treg cells in CD4+ T cells were (9.78±2.15)%, (7.23±1.72)%, (5.69±1.67)% , and (4.62±0.97)% in group A, B,C and D,respectively, group A, B and C were higher than (4.43±0.97)% of control group(P<0.05), but group D had no significant difference compared with control group(P>0.05). HIV RNA levels were (0-5.36)×107copies/mL in HIVinfected patients, the average levels of HIV RNA were (5.36±1.21)×106copies/mL ; HIV RNA levels in group A,B,C and D were(6.24±1.36)×107copies/mL,(4.36±1.02) ×106 copies/mL,(5.78±1.67)×104copies/mL,and (3.54±1.25) ×103copies/mL ,respectively,there was significant difference between each group(P<0.05), HIV RNA levels were negatively correlated with CD4 +T cells count. ConclusionHIV infection can result in the decrease of Th17 and CD4+T cells, imbalance of Th17/Treg cells, and negative correlation with HIV RNA levels.

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引用本文

吴敏泉,罗艳,何艳,等. HIV感染者Th17和Treg细胞的变化及意义[J]. 中国感染控制杂志,2011,10(4):252-255.
WU Minquan, LUO Yan, HE Yan, et al. Significance of change in Th17 and Treg cells in HIVinfected patients[J]. Chin J Infect Control, 2011,10(4):252-255.

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  • 收稿日期:2011-05-23
  • 最后修改日期:2011-06-12
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  • 在线发布日期: 2011-07-30
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