Abstract:ObjectiveTo evaluate the effect of suppressive oligodeoxynucleotides (Sup ODN) on expression of Tbet, pSTAT4 and pSTAT6 of CD4+T spleen lymphocytes in vitro.MethodsThe spleen lymphocytes of BALB/c mice were isolated and then CD4+T cells were purified with immunomagnetic microbeads(positive selection),the purification was analysed by flow cytometry.The coculture of CD4+cells, antiCD3ε, antiCD28, IL12 and Sup ODN or control oligodeoxynucleotides(Con ODN) was performed for 72 hours,the nucleoprotein was extracted with regular procedure. Expression of pSTAT4(phosphorylated STAT4),pSTAT6,and Tbet in nucleoprotein were detected by Westernblot.ResultsCompared with Con ODN and PBS treated group, expression of Tbet and pSTAT4 in Sup ODN treated group decreased significantly (P<0.05), while expression of pSTAT6 in Sup ODN treated group increased significantly (P<0.05).ConclusionSup ODN may inhibit Th1 differentiation of mice CD4+T cell by downregulating expression of pSTAT4 and Tbet, and promote Th2 differentiation by upregulating expression of pSTAT6.