ObjectiveTo evaluate the effectiveness of refined management on the prevention and control of multidrugresistant organisms (MDROs). MethodsRefined management concept and methods were adopted to standardize  all aspects of  management of MDROs, healthcareassociated infection (HAI) caused by MDROs in 2012 (before refined management) and 2013 (after refined management ) was compared.ResultsThe percentage of MDRO infection in 2013 was lower than 2012  (36.65%［70/191］ vs 48.44%［62/128］）; rate of HAI reporting , contact precautions implementation, and hand hygiene compliance in 2013 were all higher than  2012 ( 80.00% vs 32.25% ; 70.16% vs 28.13%; 75.86% vs 44.09%, all P＜0.05）. ConclusionImplementation of refined management can improve health care workers’ awareness of prevention and control of MDRO HAI , and promote continuous improvement of HAI management quality.