ObjectiveTo analyze the isolation rates and antimicrobial resistance of Acinetobacter baumannii(AB) from intensive care unit(ICU) between 2010 and 2013, and provide evidence for clinical antiinfective therapy.MethodsThe isolation and antimicrobial resistance of AB from ICU between 2010 and 2013 were analyzed retrospectively.ResultsA total of 1 413 pathogenic strains were isolated, 556(39.35%) of which were AB, isolation rates in each year were 39.45%,41.35%,29.44%,and 40.53% respectively. AB were mainly isolated from lower respiratory tract (75.72%). Antimicrobial susceptibility testing results showed that AB had low resistance rates to cefoperazone/sulbactam（5.85%）and amikacin （17.45%）；detection rates of multidrugresistant and extensively drugresistant AB increased from 9.63% and 3.70% to 42.50% and 31.88%，respectively（both P＜0.001）.ConclusionAB is the common pathogen in ICU, antimicrobial resistance is serious, isolation of multidrugresistant and extensively drugresistant AB increased year by year； intensifying the monitoring of drug resistance is helpful for the treatment and prevention of AB infection.