ObjectiveTo explore the cost of healthcareassociated infection (HAI) management in a tertiary firstclass hospital, provide data support for costeffectiveness and costbenefit analysis of HAI management, and provide scientific evidence for the rational allocation of hospital resources. MethodsMicrocosting study was used to calculate the direct cost of the department of HAI management by collecting the quantity and unit price of each item. ResultsThe total cost of HAI management in this hospital in 2013 were about ￥870 000, including human cost ￥790 000, depreciated fixed assets ￥34 501, lowvalue consumption goods ￥3 800, publicity and training ￥33 600, office consumables ￥5 208; average cost were ￥12.16 per person and ￥529.69 per bed.ConclusionHuman cost is the main cost in HAI management in this hospital.