ObjectiveTo compare the effect of GammaTM wet wipes and 75% alcohol on the disinfection of  Xray protective clothing， and provide reference for choosing  rational disinfection methods.Methods62 pieces of X  ray protective clothing were selected, 30 were from operating rooms, 32 from catheterization  room.  Protective clothing  were randomly divided into trial group and control group, each had 31 pieces. Trial group disinfected clothing with GammaTM wet wipes, control group disinfected  clothing with 75% alcohol, disinfection effect, drying time, and cost  between  both groups were compared.ResultsAverage bacterial colony counts in trial group and control group before disinfection were 2.38 CFU/cm2  and   2.36 CFU/cm2  respectively, qualified rates were 80.64% and 83.87%  respectively. Bacteria were not detected in both groups after disinfection. The average drying time in trial group was shorter than control group（［352.35±9.93］ s  vs［676.13±10.62］ s，t=123.98，P＜0.01）. The cost of disinfecting Xray protective clothing in trial group was 29.60% less than control group. ConclusionEffect of GammaTM wet wipes and 75% alcohol on disinfecting  Xray protective clothing are both reliable, compared with 75% alcohol, GammaTM wet wipes are without  corrosion,  have shorter drying time, and save more cost.