ObjectiveTo study the intervention effectiveness of intensive training in medical interns’ occupational exposure protection. MethodsMedical interns in seven colleges and universities in Wuhan between 2011 and 2015 were chosen and divided into control group (675 medical interns in 2011-2013,comprehensive training about healthcareassociated infection control was conducted for once before internship) and intervention group (682 medical interns in 2014-2015, hospitaldepartmenttutor three steps of intensive training was conducted),  the occurrence of occupational exposure and exposure knowledge awareness between two groups were compared. ResultsThe awareness rate of knowledge about occupational exposure protection and the implementation rate of protective measures in intervention group were both  higher than  control group （all P＜0.05）. Incidence of occupational exposure in intervention group was lower than control group (21.70%［n=148］ vs 65.33%［n=441］, χ2=262.91，P＜0.01）；percentage of interns who conducted active serological virus detection and intensified vaccination of hepatitis B virus were both higher than control group (14.66%［n=100］ vs 2.96%［n=20］;  11.73%［n=80］ vs 2.67%［n=18］,respectively). Occupational exposure before and after intervention occurred most frequently when interns were preparing medicine, occupational exposure reporting rate in intervention group was higher than control group (72.97%［108/148］ vs 50.11%［221/441］, χ2=52.78，P＜0.01）.ConclusionThe intensive training of occupational exposure protection among medical interns can improve the awareness and skills of occupational protection, and reduce the occurrence of occupational exposure.