ObjectiveTo understand the homology of carbapenemresistant Acinetobacter baumannii (CRAB) isolated from hospitalized patients in a hospital, and provide evidence for the prevention of CRAB spread in hospital.MethodsAntimicrobial susceptibility testing of 62 strains of CRAB isolated from all kinds of clinical specimens from hospitalized patients between August 2015 and November 2016 was performed, homology and epidemic characteristics were analyzed by pulsedfield gel electrophoresis (PFGE).Results62 strains of CRAB were mainly from sputum specimens (88.71%), all were resistant to ceftazidime, cefepime, imipenem, meropenem, and ciprofloxacin, resistance rate to levofloxacin was the lowest (25.81%). 62 strains of CRAB were divided into 14 different types (A-N), type B, D, E, J, and M only contained 1 strain respectively, type F contained 5 subtypes, type A, G, H, and K had 3 subtypes respectively, type C and I had 2 subtypes respectively. Clinical data of the main cloned strains were analyzed, 32 strains (51.61%) were isolated from patients in intensive care unit (ICU), and 12 strains (19.35%) from patients who had ever stayed in ICU.ConclusionThere are two forms of Acinetobacter baumannii prevailed in hospital, which are external and internal spread, infection control should be strengthened.