Objective To study the effect and duration of point-of-use filters on the improvement of endoscopic final rinse water quality. Methods The final rinse water end at the gastroscope manual cleaning workstation in the Endoscopy Centre of the First Affiliated Hospital with Nanjing Medical University was selected to install a tap terminal filter; five specimens of final rinse water were collected consecutively before the installation, immediately after the installation, and 1-11 weeks after the installation. At each sampling time, the staff responsible for clea-ning and disinfecting were asked whether the flow rate of discharged water could satisfy the working demand; the final rinse water was inoculated on R2A culture medium with membrane filter method, bacterial colony forming unit (CFU) was calculated after 30℃ incubation for 5 days. Results The qualified rates of endoscopic final rinse water before point-of-use filter installation was 0, immediately after and 1-9 weeks after installation were both 100%, 10 and 11 weeks after installation were 80.0% and 20.0%, respectively. The mean CFU of endoscopic final rinse water before point-of-use filter installation was 102 CFU/100 mL, immediately after and 1-9 weeks after installation were both ≤2 CFU/100 mL, 10 and 11 weeks after installation were 8 and 18 CFU/100 mL, respectively. The feedback from the cleaning and disinfection staff before installation, immediately after installation, and 1-11 weeks after installation indicated that the flow rate of discharged water gradually slowed down over time, but could still meet the work requirements. Conclusion The point-of-use filter can quickly and effectively improve the quality of endoscopic final rinse water, with use duration of up to 9 weeks after installation; Its biggest advantage is that it can serve as the final barrier to all integrated measures, playing a supplementary role in case of any problems occu-rring in the front-end process, and ensuring the microbial quality of the final rinse water to the greatest extent possible.